1. Introduction of RVvictor

The goal of RVvictor is to provide a complex RNA-directed host interaction network and more functional annotation information for this network to identify the development of ant-viral strategy to address current and future threats. In RVvictor, you can obtain the following information: First, providing, for the first time, the interaction data between virus RNA & host protein, a total of 14,789 INTPROs were manually collected and explicitly provided in RVvictor, which came from 159 virus RNAs of 98 viruses/strains and 3,473 proteins of 23 host organisms. Second, offering the most comprehensive RNA-directed interaction data of RNA virus’ translation and replication among existing databases by collectively describing all three types of virus RNA-directed molecular interactions. A total of 116,135 virus RNA-directed interactions (94875 pairs of INTmRNA and 6471 pairs of INTncRNA) were manually collected and described in our RVvictor, which originated from 154 virus RNAs of 98 viruses/strains and 24,027 proteins/mRNAs/ncRNAs of 46 organisms. Third, enabling the functional annotations of all virus RNAs in RVvictor based on the enrichment analyses of their gene/protein targets. All enrichment results were explicitly illustrated using various tables & graphs of signaling pathways, gene ontologies, and disease classes. Moreover, both the virus species and host organisms collected to RVvictor were diverse, which include a variety of well-known RNA viruses (such as: HIV, SARS-CoV-2, HCV, Ebola, Dengue, Zika, West Nile, Influenza A, Epstein Barr, and Sindbis) and various kinds of popular organisms (such as: human, monkey, mouse, rat, pig, chicken, and bovine).

2. Search for viral RNA-host molecular interactions by Virus

2.1 Search by virus name

For example, if users want to know detailed information on the interaction between any viral RNA and the host proteins, (1) Users can search by entering a virus name in the search box on the home page.

(2) Users can also search by clicking the different viruses on the homepage.

(3) If users want to search for detailed information on the interaction between any viral RNA and the host proteins by virus name, users can enter the virus name in the “Search for Virus RNA Entries” box; Users can search for viruses by selecting virus names in the “Search for Virus RNA Entries by Virus Name” box; Users can select the virus family and virus name in the “Search for Virus RNA Entries by the virus family”

2.2 Resulting webpage of Virus RNA

The resulting webpage displays profiles of all the strain regions and strains general information on the virus. By clicking the “Virus info” button, detailed information on virus strain regions and host protein interaction information can be recursively searched.

2.3 Detailed information on Virus page

The virus page contains mainly “Virus General Information”, “Virus virion and genome Information”, “Virus-RNA -- Host-Protein Interaction”, “Virus-RNA -- Host-ncRNA Interaction”, “Virus-ncRNA -- Host-Gene Interaction”, “Virus-ncRNA -- Host-ncRNA Interaction”, “Virus Sequence Information”.

Users can click the “RNA Info” button on the “Virus-RNA -- Host-Protein Interaction” page to search for the detailed information of viral RNA, and click the “Pro Info” button to search for the detailed information of host proteins.

Users can click the “RNA Info” button on the “Virus-RNA -- Host-ncRNA Interaction” page to search for detailed information of viral RNA, and click the “HncRNA Info” button to search for detailed information of host ncRNA.

Users can click the button “VncRNA Info” on the “Virus-ncRNA -- Host-Gene Interaction” page to search for detailed information of virus ncRNA, and click the button “Gene Info” to search for detailed information of host gene.

Users can click the button “VncRNA Info” on the “Virus-ncRNA -- Host-ncRNA Interaction” page to search for the detailed information of virus ncRNA, and click the button “HncRNA Info” to search for the detailed information of host ncRNA.

2.4 Detailed information on Virus RNA page

The virus RNA page contains mainly “Detail of Virus RNA”, “Full list of protein interacting with the 3' UTR of this Strain”, and “Function GO Enrichment analysis of those proteins interacting with the 3' UTR of this Strain” and “Function KEGG Enrichment analysis of those proteins interacting with the 3' UTR of this Strain”.

3. Search for Virus RNA-Host Protein Interactions by Host Protein in RVvictor

3.1 Search by Host Protein Name

Virus RNA-interacting host proteins can be searched by entering the protein name in the “search-box” on the main page and in the “Search for protein entries” on the search page.

3.2 Search by Subcellular Localization

In “Search for Protein Entries by Protein Subcellular Localization”, select a cellular subcellular localization, and then select a related protein name.

3.3 Search by KEGG Pathways

In “Search for Protein Entries by KEGG Pathways”, select the name of a KEGG pathway, and then select the name of a protein related to this pathway.

3.4 Detailed information on the host protein page

The resulting page presents detailed information on all host proteins for viral RNA interactions. By clicking the “Pro Info” button, the detailed information page of this RNA interaction protein will be displayed.

Details of host protein page contain mainly “Host Protein General Information”, “Full list of Virus RNA interaction with this Protein”, “Potential Drug Target Information of this Protein”, and “Protein Sequence Information”.

4. Search for Virus RNA-Host ncRNA interactions by Virus RNA

4.1 Search by Virus Name

If users want to search for detailed information on the interaction between any viral RNA and the host ncRNA by virus name, users can enter the virus name in the “Search for Virus RNA Entries” box; Users can search for viruses by selecting virus name in the “Search for Virus RNA Entries by Virus Name” box; Users can select the virus family and virus name in the “Search for Virus RNA Entries by the Virus Family”.

4.2 Search by Virus RNA Region

If users want to know detailed information on the interaction between any of the virus RNA regions and host ncRNA, users can enter the virus RNA region in the “Search for Virus RNA Entries” section on the virus search page.

4.3 Resulting webpage of Virus RNA

The resulting webpage displays profiles of all the strain regions and strains general information on viruses. By clicking the “Virus Info” button, detailed information on virus strain regions and host ncRNA interaction information can be recursively searched. By clicking the “RNA Info” button, detailed viral RNA information can be recursively searched.

4.4 Detailed information on virus RNA page

The virus RNA page contains mainly “Virus RNA General Information”, “Full list of Host ncRNAs interacting with the Virus RNA”, “Host ncRNA GO Enrichment”, “Host ncRNA KEGG Pathway Enrichment” and “Virus RNA Sequence Information”.

5. Search for Virus RNA-Host ncRNA Interactions by Host ncRNA

5.1 Search by Host ncRNA Name

If users want to know detailed information on the interaction between any of the virus RNA and host ncRNA, users can search by entering the host ncRNA name in the “search-box” on the main page or in the “Search for Host ncRNA Entries” field on the search page.

In addition, users can also search for viral RNA-host ncRNA interactions by selecting a host species and ncRNA symbol in the “Search for Host ncRNA Entries by the Host Species Name” on the search page.

5.2 Search by Host ncRNA Name

The resulting web page shows detailed information on viral RNA interaction host ncRNA. By clicking the “Detail Info” button, the detailed information page of host ncRNA will be displayed.

5.3 Detailed information on the host ncRNA page

Details of host ncRNA page contain mainly “Detail of Host ncRNA” and “Full list of Virus RNA Regions Interacting with this Host ncRNA”.

6. Search for Virus ncRNA-Host Gene interactions by Virus ncRNA

6.1 Search by Virus ncRNA

If users want to search for detailed information on the interaction between any viral ncRNA and the host gene by virus ncRNA, users can enter the Virus ncRNA in the “Search for Virus ncRNA Entries” box.

6.2Search by Virus Name

Users can Search for viruses by selecting virus names in the “Search for Virus ncRNA Entries by Virus Name” box.

6.3 Resulting webpage of Virus ncRNA

The resulting webpage displays profiles of all the ncRNA regions and strains general information on virus. By clicking the “ncRNA Info” button, detailed information on virus strains regions and host gene interaction information can be recursively searched. By clicking the “Virus Info” button, detailed viral RNA information can be recursively searched.

6.4 Detailed information on Virus ncRNA page

The virus RNA page contains mainly “Details of Virus ncRNA”, “Full list of Host Gene interacting with the Virus ncRNA”, “Host Gene GO Enrichment”, “Host Gene KEGG Pathway Enrichment”.

7. Search for Virus ncRNA-Host Gene Interactions by Host Gene

7.1 Search by Host Gene

If users want to search for detailed information on the interaction between any viral ncRNA and host gene by host gene, users can enter the host gene name in the “Search for Host Gene Entries” box on the search page or select the host species and host gene names in the “Search for Host Entries by the Host Species Name”.

7.2 Search by species category

If users want to search for detailed information on the interaction between any viral ncRNA and host gene by species category, users can search by selecting a host species in “Search for Host Gene Entries by Host Species” on the search page.

7.3 Resulting webpage of host Gene

The resulting web page shows detailed information on viral ncRNA interaction host gene. By clicking the “Detail Info” button, detailed information on Virus ncRNA and host Gene interaction information can be recursively searched.

7.4 Detailed information on Host Gene page

The host gene page contains mainly “Details of Host Gene”, “Full list of Virus ncRNAs interacting with this Host Gene”.

8. Search for Virus ncRNA-Host ncRNA interactions by Virus ncRNA

8.1 Search by Virus ncRNA

If users want to search for detailed information on the interaction between any viral ncRNA and the host ncRNA by virus ncRNA, users can enter the virus ncRNA name in the “Search for Virus ncRNA Entries” box.

8.2 Search by Virus Name

If users want to search for detailed information on the interaction between any viral ncRNA and the host ncRNA by virus name, users can search for viruses by selecting virus names in the “Search for Virus ncRNA Entries by Virus Name” box

8.3 Resulting webpage of Virus ncRNA

The resulting webpage displays profiles of all the virus ncRNA regions and strains and general information on virus. By clicking the “ncRNA Info” button, detailed information on virus strain regions and host ncRNA interaction information can be recursively searched. By clicking the “Virus Info” button, detailed viral information can be recursively searched.

8.4 Detailed information on Virus ncRNA page

The virus ncRNA page contains mainly “Details of Virus ncRNA”, “Full list of Host ncRNA interacting with the Virus ncRNA”, “Host ncRNA GO Enrichment”, “Host ncRNA KEGG Pathway Enrichment”.

9. Search for Virus ncRNA-Host ncRNA Interactions by Host ncRNA

9.1 Search by Host ncRNA Name

If users want to know detailed information on the interaction between any of the virus ncRNA and host ncRNA, users can search by entering the host ncRNA name in the “Search for Host ncRNA Entries” field on the search page.

9.2 Search by Species Category

If users want to search for detailed information on the interaction between any viral ncRNA and host ncRNA by species category, users can search by selecting a host species in “Search for Host ncRNA Entries by Host Species” on the search page.

9.3 Search by Host ncRNA Symbol

If users want to search for detailed information on the interaction between any viral ncRNA and host ncRNA by host ncRNA symbol, users can search by selecting the species category and an ncRNA symbol in the search “Search for Host ncRNA Entries by the Host Species Name” box.

9.4 Resulting webpage of Host ncRNA

The resulting webpage displays profiles of all the Host ncRNA regions general information. By clicking the “Detail Info” button, detailed information on host ncRNA information can be recursively searched.

9.5 Host ncRNA

The Host ncRNA page contains mainly “Details of Host ncRNA”, “Full list of Virus ncRNA interacting with the Host ncRNA”.

10. Download Page

On the download page, users can download all the complete data in the database, including “The pair information of Virus RNA-Host Protein interactions”, “Virus and virus RNA general information Downloads”, “Host proteins associated with interaction Downloads”, “Host messenger RNA associated with interaction Downloads”, “Host ncRNA associated with interaction Downloads”.